Extended Data Fig. 9: Onset of acute GI-GvHD shifts intestinal bacterial and viral communities and impacts IMM expression profiles. | Nature Cancer

Extended Data Fig. 9: Onset of acute GI-GvHD shifts intestinal bacterial and viral communities and impacts IMM expression profiles.

From: Bacteria and bacteriophage consortia are associated with protective intestinal metabolites in patients receiving stem cell transplantation

Extended Data Fig. 9: Onset of acute GI-GvHD shifts intestinal bacterial and viral communities and impacts IMM expression profiles.

a) Quantification of the bacterial and fungal load by 16S and 28S rDNA copy numbers per gram of stool stratified by patients diagnosed with GI-GvHD (GI-GvHD, red) vs control allo-SCT patients (No GI-GvHD, blue). The box ranges from the 25th to 75th percentiles. The line in the middle of the box is plotted at the median. The whiskers are drawn down to the minimum and maximum. Samples outside the 1.5-fold IQR were regarded as outliers. Significance by two-tailed Wilcoxon rank sum test adjusted for multiple comparisons via the Benjamini & Hochberg procedure. Each individual patient is plotted as a point superimposed on the graph. Number of patients: for 16S & 28S n = 22 vs n = 37, corresponding to ‘No GI-GvHD‘ vs ‘GvHD‘, respectively. b) Intestinal fungal alpha diversity (Richness, Inverse Simpson’s diversity index) stratified by patients diagnosed with GI-GvHD (GI-GvHD) vs control allo-SCT patients (No GI-GvHD). Plots, numbers and statistics as in a). c) Beta diversity analysis illustrating changes in bacteriome, fungome and virome in patients with GI-GvHD vs control allo-SCT patients. For bacteriome and fungome, beta diversity was calculated by weighted UniFrac. For virome, beta diversity was calculated by Bray-Curtis dissimilarity. Distances were projected in PCoA. Bacteriome: Comparison between patients with GI-GvHD vs No GI-GvHD is significant (p = 0.026 by one-side pairwise Adonis test). Virome: Comparison between patients with GI-GvHD vs No GI-GvHD is significant (p = 0.03 by PERMANOVA test). d) Levels of indicated microbiota-derived metabolites stratified by patients diagnosed with GI-GvHD vs control allo-SCT patients. Significance by two-tailed Mann-Whitney test. In the scatter-dot plots, the box is plotted at the mean. Error bars indicate standard deviation. Each individual patient is plotted as a point superimposed on the graph. The number of patients per group are indicated in the legend.

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