Extended Data Fig. 7: GSK3 inhibition induces cell death in TIS liver cancer cells.
a, Relative cell numbers of Huh7 cells treated with different doses of BIO and CHIR99021 for 72 hours. b, Representative crystal violet staining of control and FIS Huh7 and Hep3B cells treated with indicated doses of CHIR98014 for 6 days. c, Representative crystal violet staining of vehicle control and TIS Huh7 cells treated with indicated doses of LY2090314 for 6 days. d, Representative crystal violet staining of Huh7-sgR26, Huh7-sgMAT2A-1 and Huh7-sgMAT2A-7 cells treated with 0.5 µM LY2090314 for 6 days. e, Representative crystal violet staining of Huh6 cells treated with indicated drug combinations for 6 days. Data presented as mean ± s.e.m. of three independent experiments; statistical significance was determined by a two-tailed Student’s t-test (a). Experiments were repeated three times independently, with similar results (b-e).
