Extended Data Fig. 6: Colocalization of onco-fetal cells in Stereo-seq data.

a, Spatial visualization of onco-fetal niche (upper) and core-ligands score (bottom) in HCC03 analyzed by Stereo-seq at bin 100 resolution. b, Boxplot showing the expression of 10 core ligands driving onco-fetal reprogramming in bins with different enrichment patterns of onco-fetal niche. c, Spatial visualization of selected core-ligands expression in HCC03 analyzed by Stereo-seq at bin 100 resolution. d-g, Spatial visualization of CXCL12-CXCR4 and DLL4-NOTCH3 ligand-receptor pairs expression. h, Boxplot showing the distance quantification between onco-fetal cells and either onco-fetal cells or other non-onco-fetal (Non-OF) cells. A distance of 1000 units is ∼0.16454 mm. POSTN⁺ CAF, n = 103 cells; PLVAP⁺ EC, n = 90 cells; FOLR2⁺ TAM, n = 28 cells; Non-OF Fibroblast, n = 472 cells; Non-OF EC, n = 103 cells; Non-OF Myeloid, n = 198 cells. For a-g, HCC01, n = 36,776 bins; HCC03, n = 23,941 bins. For b and h, Center line indicates the median value, lower and upper hinges represent the 25th and 75th percentiles, respectively, and whiskers denote 1.5× interquartile range. *P < 0.05, **P < 0.01, ***P < 0.001. Two-sided Wilcoxon test. For c-g, *P < 0.05, **P < 0.01, ***P < 0.001. Spatial cross-correlation test.