Extended Data Fig. 10: Associations among onco-fetal ecosystem, ABRS genes and Tregs.
a, Boxplots showing the expression of ABRS genes in scRNA-seq data (left), whose expression patterns are also shown in heatmap (right). b, Boxplots showing the expression of the Treg signature in scRNA-seq data (left), whose expression patterns are also shown in heatmap (right). c, The correlation between ABRS score and Treg signature in GO30140 and IMbrave150 cohorts. Linear regression and Pearson’s correlation. d, The Kaplan–Meier curves of PFS in GO30140 stratified by median expression score of the Treg signature. Multivariate Cox regression. HR, hazard ratio. P-value was determined by Kaplan–Meier survival curves and log-rank test. *P < 0.05, **P < 0.01, ***P < 0.001. e, Boxplots showing the onco-fetal score in patients with high or low levels of ABRS. f, The correlation between ABRS score and onco-fetal cell signatures in GO30140 and IMbrave150 cohorts. Linear regression and Pearson’s correlation. g, Boxplots showing the expression levels of onco-fetal cell signatures in patients with high or low levels of the Treg signature. h, Boxplots showing the onco-fetal score in patients with high or low levels of Treg signature. i, Boxplots showing the expression levels of EMT-like and non-EMT signatures in patients with high or low levels of ABRS score and the Treg signature. j, Schematic of the complete treatment process and gadolinium enhanced MRI images of M311 responder in M0, M11 and M16. M refers to month. k, Spatial visualization of Treg, EMT-like and non-EMT signatures using neighborhood index (NI) in M311 (responder) and M316 (non-responder). For a,b, n = 72,330 cells. For c-i, In GO30140, ABRS low, n = 43 patients; ABRS high, n = 43 patients; Treg low, n = 43 patients; Treg high, n = 43 patients. In IMbave150, ABRS low, n = 60 patients; ABRS high, n = 59 patients; Treg low, n = 60 patients; Treg high, n = 59 patients. For e and g-i, center line indicates the median value, lower and upper hinges represent the 25th and 75th percentiles, respectively, and whiskers denote 1.5× interquartile range. *P < 0.05, **P < 0.01, ***P < 0.001. Two-sided Wilcoxon test.
