Extended Data Fig. 9: Sirpa-/- myeloid cells enhance the activation of T lymphocytes.
a, b. Expression of differentially expressed genes in CD8+ T cell (a) or CD4+ T cell (b) between WT mice and Sirpa-/- mice in AOM/DSS-induced CRC (Wilcoxon test, two-sided). c-d. Tumor loads (c) and tumor numbers (d) were measured in AOM/DSS-induced WT mice and Sirpa-/- mice treated with CD4+ or CD8+ antibody, WT (n = 9 mice), Sirpa-/- (n = 6 mice), WT + αCD4 (n = 5 mice), Sirpa-/- + αCD4 (n = 3 mice), WT + αCD8 (n = 3 mice), Sirpa-/- + αCD8 (n = 4 mice). e. The L–R pairs in cell–cell contact signaling from TAM, mMDSC, cDC1 and cDC2 (sender) to other cell types (receiver) in AOM/DSS-treated WT mice and Sirpa-/- mice calculated by CellChat. Line width indicates signal strength and line color is the same as signaling sender. f. The dominant sender and receiver cell types of cell–cell contact signaling network calculated by CellChat. Count represents the number of L–R pairs. g. The CD8+ T cells proliferation suppressed by WT (n = 3 samples) or Sirpa-/- (n = 3 samples) gMDSCs at different ratios. All samples were isolated from independent mice Result (c, d, g) are from one independent experiment. Error bars show mean ± s.e.m. Unpaired Student’s two-tailed t-test was used for c, d, g.
