Extended Data Fig. 3: The basic information of Sirpα knockout mice and its baseline immune profile.
a. Scheme of generation of Sirpa−/− mice. b, c. Sirpα protein in WT BMDMs and Sirpa−/− BMDMs were detected by Western blot (b) and FACS (c). d. Expression level of Sirpa, Sirpb1 and Sirpd in WT BMDMs (n = 3 samples) and Sirpa−/− BMDMs (n = 3 samples) were measured by RT–qPCR. e. Comparison of myeloid cell subsets in intestine between WT mice (n = 7 samples) and Sirpa−/− mice (n = 7 samples). f. Comparison of T cell subsets in intestine between WT mice (n = 7 samples) and Sirpa-/- mice (n = 6 samples). g. Comparison of fraction of NKT cells, NK cells, total myeloid cells, total T cells and B cells in all intestinal immune cells between WT mice (n = 7 samples) and Sirpa-/- mice (n = 7 or 6 samples). h-i. The gating strategies of myeloid cells (h) or lymphocytes (i). All samples were isolated from independent mice. Results (b, c) are from one representative of three independent experiments. Results (d-g) are from one independent experiment. Error bars show mean ± s.e.m. Unpaired Student’s t-test was used for d-g.
