Extended Data Fig. 2: Autotaxin-LPA signaling regulates pancreatic cancer immune contexture including suppression of eosinophil infiltration. | Nature Cancer

Extended Data Fig. 2: Autotaxin-LPA signaling regulates pancreatic cancer immune contexture including suppression of eosinophil infiltration.

From: Autotaxin–lysolipid signaling suppresses a CCL11–eosinophil axis to promote pancreatic cancer progression

Extended Data Fig. 2

a, Spectral flow cytometry gating strategy used for isolation of pure FACS sorted eosinophil populations from Dox inducible control and shEnpp2 tumors. b, Quantification of the flow cytometry-based immune profiling of Dox inducible Enpp2 knockdown tumors. Data are presented as mean ± SEM (n = 3 tumors per group). ns= not significant, **P= 0.0032, ****P< 0.0001 by two-way ANOVA. c, Flow cytometry gating strategy used for isolation of eosinophil populations from FC1245 sgCtrl and sgEnpp2 tumors. d, Immunohistochemical staining and quantitation of MBP-1 in FC1245 sgCtrl and sgEnpp2 tumors. Scale bar = 100 mm. Data are shown as mean ± SEM (n = 14 ROIs from 4 tumors per group). *P= 0.0168 by two-tailed unpaired t-test.

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