Extended Data Fig. 5: Lung endothelial cells express canonical and noncanonical Wnt ligands.
From: Lung endothelium exploits susceptible tumor cell states to instruct metastatic latency
a, Mean summed expression of Wnt-ligands in all capillary EC, b, in filtered aCap or c, in filtered gCap pseudo-bulks of biological replicates. Data are presented as mean values +/− s.d., p values were calculated by one-way ANOVA with Tukey post-test. n.s., not significant. n = 3-4 mice. d, Expression profile for detected Wnt-ligands in individual lung EC of the combined scRNAseq dataset. e, Expression profile of the 4 top expressed Wnt ligands split by timepoint and by EC subtype. f, Correlation of noncanonical Wnt gene scores and canonical Wnt gene scores in cells of the individual tumor cell clusters are shown. P value and r value by Pearson correlation are shown. g, Violin plots of scored gene expression of genes associated with canonical Wnt-signalling (left panel), and noncanonical Wnt-signalling (right panel) in individual TC clusters. h, Schematic of experiment. TC were treated 2 weeks in vitro with WNT5A or PBS control. Quantification shows percentage of extravasated tumor cells 1.5 days postinjection of control or WNT5A treated TC. n = 6 mice. i, Percentage of latent tumor cells 3.5 days postinjection of control or WNT5A treated TC, and j, metastatic outgrowth, calculated as fold change in TC abundance at day 3.5 compared to the mean of the respective day 1.5 timepoint. n = 5 mice. k, Relative expression of key EMT-associated genes Klf4, Zeb1, Snai1, Vim, and Twist1 in cultured 4T1 control or WNT5A treated cells. Target gene expression was normalized to Actb and relative expression to 4T1 cells was calculated using the 2-ΔΔCt-method. n = 3 independent experiments. h-k, Data are presented as mean values +/− s.d., p value by two-tailed t-test are shown. n.s., not significant.
