Extended Data Fig. 6: Angiocrine Wnt is a crucial instructor of metastatic latency.
From: Lung endothelium exploits susceptible tumor cell states to instruct metastatic latency
a, Recombination efficiency of Wls-iECKO mice. Expression of Wls was normalized to Actb and fold changes were calculated using the 2-ΔΔCt-method. Data are presented as mean values +/− s.d., p value by two-tailed t-test is shown. n = 9 mice. b, Representative images of metastatic lungs from Cre- control (upper image) and Cre+ iECKO mice (lower image) two weeks postinjection of 2 × 105 B16F10 melanoma cells (left panel) and corresponding quantification of visible metastatic foci per lung (right panel). Data are presented as mean values +/− s.d., p value by two-tailed t-test is shown. n = 13 mice. c, Primary tumor growth curves for vehicle control and LGK974 treated mice. n = 14 for control group, n = 12 for LGK974 treatment group. Data are presented as mean values +/− s.d., p values for each measurement timepoint were calculated by two-tailed t-test. n.s., not significant. d, Body weights of mice at the experimental endpoint. Data are presented as mean values +/− s.d., p value was calculated by two-tailed t-test. n.s., not significant. n = 12–14 mice. e, Representative images of primary tumor vasculature for vehicle control (upper panels) and LGK974 treated mice (lower panels). f, Corresponding quantifications of total vessel area per region of interest (left panel) and desmin coverage of CD31+ vessels (right panel). Data are presented as mean values +/− s.d., p values were calculated by two-tailed t-test. n.s., not significant. n = 11–13 mice. g, Representative images (left panel) of metastatic lungs from vehicle control (upper image) and LGK974 treated mice (lower image). Dotted white circles indicate metastatic nodules. Quantification of lung weight to body weight ratio for vehicle control and LGK974 treated mice at the experimental endpoint. Data are presented as mean values +/− s.d., p value by two-tailed t-test is shown. n = 12–14 mice.
