Extended Data Fig. 8: Wnt-agonistic treatment in vitro does not change cellular identity.
From: Lung endothelium exploits susceptible tumor cell states to instruct metastatic latency
a, EMT- and Wnt-gene scores in CTC isolated from breast cancer patients in publicly available datasets. Left panels display 252 cells, right panels display 89 cells. b, Schematic of experiment. 4T1-GFP cells were pulse treated with CHIR99021 or vehicle control overnight prior to injection (left panel). Percentage of extravasated TC 1.5 days (mid panel) and percentage of TC with dye retention 3.5 days postinjection (right panel). Data are presented as mean values +/− s.d., p values were calculated by two-tailed t-test. n.s., not significant. c, Relative expression of key EMT-inducing transcription factors Klf4, Zeb1, Snai1 and Twist1 and canonical Wnt-downstream target Axin2 in 4T1 cells pulse-treated (over-night) or reprogrammed (two-week treatment) with Wnt-agonist CHIR99021 or d, SKL2001. Target gene expression was normalized to Actb and relative expression to respective controls was calculated using the 2-ΔΔCt-method. Data are presented as mean values +/− s.d., p value by two-tailed t-test are shown. n.s., not significant. * p value < 0.05, ** p value < 0.01, *** p value < 0.001, **** p value < 0.0001.
