Fig. 4: Resolving the temporal Wnt interactome of endothelial and TCs.
From: Lung endothelium exploits susceptible tumor cell states to instruct metastatic latency
a–c, DGEA on TC pseudobulks reflecting the sort gate and biological replicate. Extravascular cells were compared with intravascular cells and LTCs with proliferative cells (full set of DEGs is shown in Supplementary Table 6). DEGs were mapped against CellPhoneDB and filtered for genes that had annotated interaction partners expressed in the lung EC scRNA-seq dataset. Y axis shows log2 FC of DEGs for the latent versus proliferative comparison and x axis shows the log2 FC of the extravascular versus intravascular comparison. Blue and red backgrounds indicate enrichment of the gene for the extravasation–latent and intravascular–proliferative trajectory, respectively. Dot color represents significance: red indicates genes significantly differentially expressed for both latent–proliferative and intravascular–extravascular comparison; blue indicates genes significantly differentially expressed for latent–proliferative but not intravascular–extravascular comparison; and orange indicates genes that are significantly differentially expressed only for intravascular–extravascular comparison. Dot size indicates percentage of TCs with detectable gene expression in the scRNA-seq dataset (Pct. expressed). a, Annotated differentially expressed TC-derived ligands. b, Annotated differentially expressed TC receptors. c, Supervised analysis of Wnt receptors expressed in TCs. log2 FC and P values were computed in DESeq2 using the Wald test. Genes with adjusted P < 0.05 were considered significant. d, Expression pattern of differentially expressed Wnt receptors on the trajectory graph.
