Extended Data Fig. 2

a. DNA sanger sequencing of the PIK3CG and PIK3R5 genomic regions targeted by the CRISPR-Cas9 PIK3CG- and PIK3R5-directed guides in OCI-AML2 cells. Sequences were aligned using TIDE online tool to determine the relative efficiency of each sgRNA. Repeated twice with similar results. b. Bioluminescence pictures of three representative mice from Fig. 1j injected with OCI-AML2 infected with either a non-targeting control or a PIK3CG-directed sgRNA. Median bioluminescence is depicted in radiance on days 7, 13, 18 and 22.