Extended Data Fig. 10: Supplementary examples of immune profiles in the multiplex immunohistochemistry cohort.
From: Tumor evolution metrics predict recurrence beyond 10 years in locally advanced prostate cancer
Each image pair shows the expression of immunofluorescence markers (right), acquired by Phenocycler Fusion, with the matched region from H&E staining (left). (A, B) Examples of immune hot regions. (C, D) Examples of regions abundant for CD20 (cyan). (E, F) Examples of regions abundant for CD68 (green). CD163 (orange) is rarely seen, suggesting that these are likely to be solely M1 macrophages. Immunohistochemistry experiments were run once following optimisation and validation.
