Fig. 6: CD8+ T cell cluster relationships within and across tissues of CT26 tumor-bearing mice following anti-TIGIT and/or anti-PD-L1 treatment.
a,b, Chord diagrams showing numbers of intraclonal pairs between clusters in dLNs (a) or tumors (b). Intraclonal pairs count all pairwise combinations of cluster phenotypes summed over all clones. Lines are shown for all intraclonal pairs between cells with different clusters, with their thickness representing the number of pairs relative to the total number of pairs constituting the full circle. Regions around the circumference without lines represent intraclonal pairs between cells with the same cluster. Red lines denote gp70+ clones; blue lines denote gp70– clones. Singleton clones do not have intraclonal pairs and are therefore not represented in this analysis. nS represents the total number of pairwise counts within the same cluster; nD is the pairwise counts between different clusters. c, Intraclonal pairs shared between dLNs, blood and tumors. Each chord diagram contains clusters from blood (Bl), dLNs and tumors, separated by gaps. Lines are shown for all intraclonal pairs between cells from different tissues. nS is the pairwise counts within the same tissue; nD is the pairwise counts between different tissues. d,e, Cluster co-occurrence links for gp70+ (d) or gp70– (e) clones in dLNs, blood and tumors, projected onto UMAP plots. UMAP plots show the cells of the given tissue and gp70 specificity for each experimental condition. Thickness of lines denotes relative strength of co-occurrence and correlates with line thickness shown in chord diagrams, with an additional multiplier of 3 for migration links between tissues. Lines within dLNs and tumors were pruned by an MST algorithm to show primary relationships. Migration lines from dLNs to blood and from blood to tumor were not subjected to MST. NL represents the total number of cells in dLNs; nB is the number of cells in blood on day 7; nT is the number of cells in tumors.
