Extended Data Fig. 4: IFN-α therapy induces TLR7 on myeloid cells in tumor stroma.
a, TLR7 immunohistochemistry staining of human skin. To the left: Representative pictures before and during IFN-α therapy. Insets show enlarged views of the marked areas. To the right: Quantification of TLR7-positive cells is presented as a percentage of total cells. Scale bar: 100 µm. In a, b n = 5 individuals. b, TLR8 staining of human skin of patients undergoing IFN-I therapy. On the left are representative pictures. Insets provide enlarged views of the marked areas. On the right is the quantification of TLR8-positive cells which is shown as percentage of total cells. Scale bar: 100 µm. c, Multiplex immunofluorescence analysis of melanoma samples from a cohort of five patients undergoing IFN-α therapy. The staining panel included antibodies against CD1a, CD1c, CD68, CD141, and TLR7. Representative images show the upper dermis of a patient before (upper panels) and during IFN-α therapy (lower panels). On the left, a full-color panel, and on the right TLR7 in combination with selected markers is shown. Insets show enlarged views of the marked areas, and highlight cells that are positive for TLR7 and/or one of the myeloid cell markers. Magnification: 10x (Zoom), Scale bar: 50 µm, Insets: 10 µm. In d n = 5 individuals. d, Quantification of TLR7-expressing cells positive for CD1a, CD1c, CD68 and CD141 was performed in the upper dermis in immunofluorescence staining’s described in (c). N.D. = not detectable. Data are plotted as dots and lines. Dots in a, b and represent individuals. P values were calculated using paired, two-tailed t-test (a, b and d).
