Extended Data Fig. 6: c-Jun modulates the immune response in DCs and macrophages downstream of TLR7.

a, c-Jun mRNA expression levels were measured in murine bone-marrow-derived DCs using qRT–PCR. Cells were pre-treated with IFN-α (6 h) and/or stimulated with IMQ over night. (n = 6 mice per group; Data are pooled from 2 independent experiments). b, Il-12a mRNA expression levels were assessed by qRT–PCR in BM-DCs as described in (a). (n = 6 mice, c-Jun^fl/fl: Control n = 5, IMQ n = 5, c-Jun^Δ/ΔMx1-Cre: IMQ n = 5, IFN-α + IMQ n = 5; n is the number of mice pooled from 2 independent experiments). c, Tlr7 and Mx1 mRNA expression levels were assessed by qRT–PCR in BM-DCs as described in (a). d, IL-12 protein levels were measured by ELISA in the supernatant of bone-marrow-derived macrophages following the indicated treatment. N.D.: Not detectable; (c-Jun^fl/fl n = 4 mice, c-Jun^Δ/ΔMx1-Cre; n = 3 mice; 1 experiment). e, Tlr7, c-Jun, and Il-12b mRNA expression levels were analyzed by qRT–PCR in tumor-infiltrating DCs (CD45⁺CD64^-CD11c⁺MHC-II⁺) that were isolated from B16-F10 tumors of mice with the indicated genotype and treated with IMQ topically and orally. Tlr7: Control group n = 5, IMQ topical &oral: c-Jun^fl/fl n = 6, c-Jun^Δ/ΔMx1-Cre n = 7, c-Jun: Control group n = 5, IMQ topical &oral group n = 7, Il12-b: Control group: c-Jun^fl/fl n = 5, c-Jun^Δ/ΔMx1-Cre n = 4, IMQ topical &oral group n = 7; n is the number of mice pooled from 2 independent experiments. f, Tlr7, c-Jun, and Il-12b mRNA expression levels were analyzed by qRT–PCR in tumor-associated macrophages (CD45⁺CD64⁺CD11b⁺), as described in (e). Tlr7 and c-Jun: Control group n = 5, IMQ topical &oral n = 7, Il-12b: Control group: c-Jun^fl/fl n = 5, c-Jun^Δ/ΔCD11c-Cre n = 3, IMQ topical &oral: c-Jun^fl/fl n = 7, c-Jun^Δ/ΔCD11c-Cre n = 5; n is the number of mice pooled from 2 independent experiments. g, Tlr7, c-Jun, and Il-12b mRNA expression levels were analyzed by qRT–PCR in sorted macrophages (CD45⁺CD64⁺CD11b⁺). Macrophages were isolated from B16-F10 tumors that were implanted in c-Jun^fl/fl and c-Jun^∆/∆CD11c-Cre mice. Tumors were treated with IMQ topically and mice received IFN-α (see Extended Data Fig. 2b). Tlr7 n = 6 mice per group, c-Jun and Il-12b: n = 6 mice per group and in the Control: c-Jun^Δ/ΔCD11c-Cre group n = 5 mice; n is the number of mice pooled from 2 independent experiments. Data are plotted as mean ± SEM. Dots in a-g represent biological replicates. P‐values were calculated using two-way ANOVA with Sidak´s post-test. (a-g).

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