Extended Data Fig. 4: Characterization of patient-derived CRC organoids. | Nature Cancer

Extended Data Fig. 4: Characterization of patient-derived CRC organoids.

From: First-in-class ultralong-target-residence-time p38α inhibitors as a mitosis-targeted therapy for colorectal cancer

Extended Data Fig. 4: Characterization of patient-derived CRC organoids.The alternative text for this image may have been generated using AI.

a, Representative pictures of H + E and immunohistochemical staining for Pan-CK and CDX2 in human CRC tissues that were used for culture generation (PDO1-4) (n = 2 biologically independent experiments). Scale bars, 100 µm. b, Representative pictures of patient-derived CRC organoid cultures (PDO1-5) stained for CK20 and CDX2 (n = 2 biologically independent experiments). Scale bars, 100 µm. c, Panel sequencing of patient-derived CRC organoids (PDO1-5). Shown are INDELS (inframe, frameshift, stop gained), SNVs (missense, stop gained) and CNVs for selected, CRC related genes. For all data, see Supplementary Tables 4–13. The experiments in Extended Data Fig. 4a, b were independently performed twice with similar results.

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