Extended Data Fig. 9: Generation of Myc-driven CRC mouse models and treatment of human CRC cultures.
a, Representative pictures of hepatic KMP metastases and H + E and immunohistochemical staining for Pan-CK and CDX2 on tumor tissue (n = 4 mice). Scale bars, 1 cm (macroscopic picture) or 100 µm (microscopic pictures). b, Representative pictures of KMP primary tumors in the caecum and KMP liver and lung metastases (with GFP imaging of tumors) and H + E and immunohistochemical staining for Pan-CK and CDX2 on tumor tissues (n = 4 mice). Scale bars, 1 cm (macroscopic pictures) or 100 µm (microscopic pictures). c-h, Cell viability analysis in the human CRC cell lines HCT-15 (c), HCT 116 (d), COLO 205 (e), LS 174 T (f), RKO (g) and HT-29 (h) upon 4 d of treatment with SKL, 1639, 2015 or DMSO (drug response curves and IC50 values, data are presented as mean values +/- SD, n = 3 cultures per condition). i, Cell viability analysis in the patient-derived organoid cultures PDO6 – PDO15 upon 4 d of treatment with 0.5 µM of SKL, 1639, 2015 or DMSO (n = 3 biologically independent experiments, data are presented as mean values +/- SD, statistical significance was calculated using an ANOVA and Tukey’s multiple comparisons test, P < 0.05). The experiments in Extended Data Fig. 9c–h and the stainings in Extended Data Fig. 9a, b were independently performed twice, the experiments in Extended Data Fig. 9i were independently performed three times, all with similar results.
