Extended Data Fig. 7: Design and function of bispecific CD19/CD22 ChTCR.

a. Schematic of CD19- and CD22- mono- and bispecific receptor constructs. b-d. Left: Representative histograms of NFAT-GFP (b), NFκB-CFP (c) and AP-1-mCherry (d) reporter expression in J76 TPR Jurkat cells expressing the indicated receptors. Middle: Frequency of NFAT-GFP (b), NFκB-CFP (c) and AP-1-mCherry (d) positive cells. Right: Geometric mean ± SD of NFAT-GFP (b), NFκB-CFP (c) and AP-1-mCherry (d) reporter genes (n = 3 independent experiments). P values calculated by two-way ANOVA. e. Representative flow plots of CD19 and CD22 expression by Nalm-6 WT, CD19⁺CD22^ko, CD19^koCD22⁺ and CD19^koCD22^ko target cells measured by flow cytometry (n = 3 independent experiments). f. Concentration of IFN-γ in supernatant after overnight co-culture of T cells expressing the indicated CARs and Bi-ChTCRs with indicated Nalm-6 cell lines. Data is shown as the mean ± SD for 3 independent experiments. P values calculated by two-way ANOVA. g. Cytotoxicity of the indicated Nalm-6 cell lines by CAR and Bi-ChTCR T cells at varying E:T ratios. (n = 3 independent experiments).

Source data