Extended Data Fig. 3: Flow cytometry gating strategy to identify dendritic cells. | Nature Cancer

Extended Data Fig. 3: Flow cytometry gating strategy to identify dendritic cells.

From: Systemic rewiring of dendritic cells by melanoma-secreted midkine impairs immune surveillance and response to immune checkpoint blockade

Extended Data Fig. 3

(a-e) Gating strategy to identify DCs and progenitor dendritic cells: (a) intratumoral DCs, cDC1s and cDC2s; (b) total cDCs (resident and migratory), cDC1s, and cDC2s in draining lymph nodes; (c) circulating cDCs, DC1s, DC2s, and pre-DCs in blood; and (d) identification of pre-DCs, common myeloid progenitors (CMPs), macrophage/dendritic cell progenitors (MDPs) and common dendritic cell progenitors (CDPs), as well as (e) mature cDCs, DC1s, DC2s in the bone marrow.

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