Extended Data Fig. 7: Functional analysis between CD19-BBz CAR-NK product groups.
a, Heatmap of representative “exhaustion,” “cytotoxicity,” and “immune potential”-related gene sets across CR, PD, and fCAR-NK group, boxplots of these three gene sets mean scores of each product (centre of box mean median, bounds of box means upper and lower quartiles, whisker means maximum and minimum, n = 2 for CR, 2 for PD, 3 for fCAR-NK), and violin plot of these gene set scores of each single cell (showing median, quartiles and extremum, n = 23644 cells from 2 CR samples, 27826 cells from 2 PD samples, 38188 cells from 3 fCAR-NK samples) and visualization on UMAP. A two-sided student’s t-test was used for every two groups. P < 0.0001. b, Module-trait relationship based on WGCNA analysis, and gene Ontology (GO) and KEGG pathway analysis of each module from WGCNA. c, The cytotoxic activities of CD19-BBz CAR-NK cells for CR + PR patients (n = 5) compared to those from SD + PD patients (n = 3) on Raji cells at the indicated effector-to-target (E/T) ratios by measured using Luciferase-based cytotoxicity assays. Data are expressed as median and quartiles (each dot plot represents 3 independent experiments and 4 technical repeats for each CAR-NK product). Two-way ANOVA (Bonferroni posttest) was used between groups. P = 0.1223. d, Cytotoxic activities of fresh CAR-NK cells compared to cryopreserved CAR-NK cells on Raji cells at the indicated effector-to-target (E/T) ratios as measured by Luciferase-based cytotoxicity assays. Data represent 3 independent experiments and 4 technical repeats, and are expressed as mean ± SEM. Two-way ANOVA (Bonferroni posttest) was used. P = 0.0012. Representative data from 1 independent experiment with 4 technical replicates.
