Fig. 6: Single-cell transcriptomic landscape of peripheral immune cells.
a, UMAP visualization of transcriptomic profiles of 51,068 cells in PBMC samples obtained from 5 patients (3 from CR and 2 from PD). Fifteen distinct clusters were identified. The dashed circles highlight immune cell subtypes exhibiting significant differences in proportions between groups. b, Distribution of each cluster between the CR and PD groups. c, Volcano diagram of DEGs between CR and PD groups in each subcluster of antigen-presenting related cells, CD4-T cells, and cytotoxic cells. d, GSVA score differences (CR versus PD, median of delta GSVA score, mean ± s.e.m.) across five gene sets (above the red dashed line indicates enrichment in CR, while below the blue dashed line indicates enrichment in PD). Dot plots represent ten subclusters which were classified into three cell types. e, Pearson correlation analyses of “CBLB-ubiquitination” gene set with antigen presentation, immunosuppression, and cytotoxicity gene sets in two different cell types. Each scatter point represents GSVA score of a subcluster of the same cell types from five samples. Correlation coefficient P value was calculated. f, Quantitative reverse transcription PCR validation of CBLB mRNA expression in patients’ PBMCs. Data are expressed as mean ± s.e.m. (n = 5 versus 4). Two-sided Student’s t-test was used. g,h, Analysis of communication among immune cells in patients with CR and patients with PD regarding TGFβ signaling (g) and MHC-II signaling (h).
