Extended Data Fig. 7: Effect of treatments on lung metastasis and kidney tumor in renal cancer mouse model. | Nature Cancer

Extended Data Fig. 7: Effect of treatments on lung metastasis and kidney tumor in renal cancer mouse model.

From: An in situ engineered chimeric IL-2 receptor potentiates the tumoricidal activity of proinflammatory CAR macrophages in renal cell carcinoma

Extended Data Fig. 7: Effect of treatments on lung metastasis and kidney tumor in renal cancer mouse model.The alternative text for this image may have been generated using AI.

(a) Representative photographs of the lungs of mice with renal cancer that were treated as indicated (top). Scale bar = 1 cm. Representative histopathological images of lung metastasis after H&E staining (middle), Scale bar = 1 mm. Photos of the lungs after fixation with picric acid to visualize pulmonary nodules (bottom), Scale bar = 1 cm. Representative pictures from n = 3 independent replicates. (b) Lung weights of kidney tumor-bearing mice after different treatments. (c) The numbers of lung nodules were counted. G1, Group 1 treated with PBS (control); G2, Group 2 treated with IL-2@gel; G3, Group 3 treated with CAR-mLNP/IL-2@gel; G4, Group 4 treated with CSR-mLNP/IL-2@gel; and G5, Group 5 treated with CAR-mLNP + CSR-mLNP/IL-2@gel; Normal, Group derived from non-tumor-bearing mice. (d) Representative photographs (scale bars, 1 cm) and H&E staining of tumor-bearing kidneys (scale bars, 500 μm) and lungs (scale bars, 1 mm) from different treatment groups. Representative pictures from n = 3 independent replicates. (e) Weights of tumor-bearing kidneys in mice after different treatments. (f) Weights of lungs in kidney tumor-bearing mice after different treatments. The data of B,C,E and F are presented as mean ± s.d. (n = 6 mice per group, biological replicates). Data are analyzed with one-way ANOVA. *P = 0.0215 for G5 versus G4 in (c), ***P = 0.0003 for CAR-mLNP + CSR-mLNP/IL-2@gel versus CAR-mLNP + CSR-mLNP/IL-2 in (e), ***P = 0.0003 for CAR-mLNP + CSR-mLNP/IL-2@gel versus CAR-mLNP + CSR-mLNP/IL-2 in (f), ns: no significance, ****p < 0.0001.(g) LPS detection in each component of CAR-mLNP + CSR-mLNP/IL-2@gel. The top part of the image presents the results of the diluted LPS standard, while the bottom part illustrates the results for the six components or their degradation products. Data are presented as mean ± s.d. (Representative pictures from 3 independent replicates) and analyzed using one-way ANOVA. ***p < 0.001, ****p < 0.0001.

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