Extended Data Fig. 2: Knockdown of TRMT6 inhibited proliferation of CRC cells.
(a) Confirmation of TRMT6 knockdown using two independent siRNAs in RKO and SW480 cells, including RNA dot blot (total RNA, 1000 ng) analysis of m1A levels in the indicated cells. (b) Knockdown of TRMT6 inhibited cell viability of RKO and SW480 cells, as assessed using CCK-8 assays (Representative results are shown, n = 6 technical replicates). (c) Knockdown of TRMT6 suppressed cell colony formation of RKO and SW480 cells (mean ± SD, n = 3 biologically independent experiments, p-values as indicated in the figure: 0.0066, 0.0065, and < 0.0001, one-way ANOVA with Tukey’s multiple comparisons test). (d) Knockdown of TRMT6 decreased the percentage of Ki-67 positive cells of RKO, SW480, and HCT116 cells (mean ± SD, n = 3 biologically independent experiments, p-values as indicated in the figure: < 0.0001, 0.0003, and 0.0006, two-tailed unpaired t-test). The representative images were shown, and a scale bar of 50 μm was used for the image. The experiments in a-d were independently performed three times, all with similar results.
