Extended Data Fig. 1: Characterization of the pressure-fused IL-2@Ca(CO3)1/2(PO4)1/3.
From: Long-acting IL-2 release from pressure-fused biomineral tablets promotes antitumor immune response
a,d, TEM images of CaC (a) and CaP (d) nanoparticles. Scale bar, 250 nm. b,e, Size distributions of CaC (b) and CaP (e) nanoparticles. c,f, XRD patterns of CaC (c) and CaP (f) tablets. g,h, XRD patterns of calcite (g) and hydroxyapatite (h) with Ca(CO3)1/2(PO4)1/3, the right figures are the magnified regions. i, SEM images of the cross section of the pressure-fused IL-2@Ca(CO3)1/2(PO4)1/3. Scale bar, 10 µm. j, SEM-EDS mapping results of the enlarged cross-section image. The dots corresponded to the elemental N from IL-2. Scale bar, 200 nm. k, SEM images of the cross section of the crystallized IL-2@Ca(CO3)1/2(PO4)1/3 tablet after 2 months. The enlarged images represented the amorphous cross-section (upper right) and the crystallized cross-section (lower right). The images indicated that the crystallized thickness of the tablet was about 90 µm. Scale bar, 30 µm (left) and 3 µm (right). l, Measurement of IL-2 contents in amorphous and crystalline phases collected from the used tablets; n = 3 samples from three independent experiments. Statistical significance was calculated via two-tailed unpaired t-test. m, Changes in the transparency of IL-2@Ca(CO3)1/2(PO4)1/3 tablet as IL-2 contents increased; n = 3 samples from three independent experiments. The total mass of IL-2@Ca(CO3)1/2(PO4)1/3 tablet was 10 mg. The loading capacity of IL-2 in the tablet was at least 3 wt.%. n, SEM image of the pressure-fused IL-2@Ca(CO3)1/2(PO4)1/3 containing 300 µg IL-2. Scale bar, 10 µm. In l,m, data are presented as mean ± s.d. In a,d, i-k, and n, experiments were repeated three times.
