Extended Data Fig. 3: CD47p/AZE-Paclitaxome-2 further enhanced drug delivery and antitumor efficacy with longest survival benefit in advanced KPC-Luc PC with metastasis.
a-c, Blood kinetics (a), tissue distribution (b) and PTX release rate in tumors (c) at 24 h in orthotopic 4T1-Luc2 tumor mouse model (n = 3 mice per group, tumors: ~300 mm3). Animals were intravenously administered once with Paclitaxome-1 or Paclitaxome-3 at 70 mg PTX/kg, respectively. d,e, Lago optical ex vivo photographs of various organs 24 h in orthotopic 4T1-Luc2 tumor mice (d, n = 3 mice per group, tumors ~300 mm3) and orthotopic KPC-Luc tumor mice (e, n = 3 mice per group, tumors ~400 mg) after i.v. injection once with DSPE–Cy5-labeled PTX/Lipo–SM. f, Lago optical ex vivo photographs of various organs 24 h in orthotopic KPC-Luc mouse model (n = 3 mice per group, tumors ~400 mg) after i.v. injection once with DSPE–Cy5, DSPE–Cy5-labeled various Paclitaxome-2. g, Representative images of extravasation and penetration for various Paclitaxome-2 within tumors from f (n = 3 tumous per group). DAPI stained nuclei (blue). PECAM1 primary antibody and Alexa Fluor 488™ secondary antibody labeled blood vessels (green). Scale bars, 50 μm. h,i, Individual tumor volume determined by a caliper (h) and average body weight (i) from Fig. 5l. j–l, Antitumor effect in orthotopic PC mice with metastasis. B6129SF1/J mice were surgically inoculated with 2 × 106 cells into their pancreas (n = 5 mice per group). When main tumors grew to ~400 mg and had discernible metastasis on day 10, animals were intravenously administered with the same dose as Fig. 5l. j, BLI of mice by Lago imaging on day 10, 17 and 24. On day 23, one mouse died from 5% dextrose. k, BLI of entire tumor burden in animal by normalization (red arrow: time of drug injection). l, Kaplan–Meier survival curves. Data are presented as average ± s.d. within a-c, i and k. One-way ANOVA with Tukey’s multiple comparisons test were used to calculate the exact P values, log-rank Mantel–Cox test was utilized to compare the survival plot in the statistical analyses.
