Fig. 5: Colocalization of CTHRC1+ fibroblasts and SLPI+ macrophages across cancer types.
a, Spatial feature plots showing the spatial localization of eFibro_CTHRC1 and Macro_ SLPI cells in two pancreatic adenocarcinoma ST datasets. b, Dot plot showing the correlation between the signature scores of eFibro_CTHRC1 (x axis) and Macro_ SLPI (y axis) cells in ST data, with color indicating the proportion of fibroblasts in each spot. The correlation was calculated using the Pearson correlation coefficient. The error band denotes the 95% confidence interval, which was calculated based on the standard error using the normal distribution. The center measure of the smooth line corresponds to the predicted values from the linear regression model. c, Scatter plots showing the correlation between the signature score of eFibro_CTHRC1 cells and all monocytes and macrophages in tissue sections, with color representing the proportion of fibroblasts in each spot and error bands indicating the 95% confidence interval. d, Immunofluorescence images showing the localization of Macro_SLPI and Macro_SPP1 cell types (DAPI, CD68, SLPI and SPP1) and eFibro_CTHRC1 cell types (DAPI and CTHRC1) in HNSC and NSCLC samples; scale bars, 50 μm. e, Genes inferred to encode the top 20 ligands separately regulate eFibro_CTHRC1 cells and SLPI+ macrophages according to NicheNet. Ligands are ranked by Pearson correlation (left). The heat maps show the expression of ligands mentioned on the left across major cell types (middle) and the top 20 ligands inferred to regulate SLPI+ macrophages (right). f, Cartoon depicting the general distribution of eFibro_CTHRC1 and Macro_SLPI populations in tumors, as well as the function. Image created with BioRender.com, with permission.
