Extended Data Fig. 4: CAR expression and transcriptomic profiling.

a, Flow cytometric data showing BCMA-4-1BBζ CAR expression on the T cells from one experiment described in Fig. 4c. b, Flow cytometric data showing ROR1-4-1BBζ CAR expression on the T cells from one experiment described in Fig. 4d-f. c-e, UMAP visualizations from single-cell RNA sequencing experiment described in Fig. 5a–e showing CD22-4-1BBζ CAR T cells ± MT-SLP-76 in separate panels at baseline (c), after 5 hours (d), and after 24 hours (e) of stimulation, from two donors. f, Volcano plots of log₂FC and log₁₀ Benjamini-Hochberg adjusted P value for the differentially expressed genes in CD22-4-1BBζ CAR T cells ± MT-SLP-76 without stimulation as determined by the Wald test using DESeq2. g, Bar chart showing the top five Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways in CD22-4-1BBζ CAR T cells with MT-SLP-76 versus CD22-4-1BBζ CAR T cells after 5 h of stimulation. Pathway enrichment analysis was performed using gene set enrichment analysis (GSEA) as implemented by fast GSEA. Pathways are ranked by –log₁₀ Benjamini–Hochberg adjusted P value, and the normalized enrichment score (NES) is shown for each term.