Extended Data Fig. 9: Probe-level filtering for spatial transcriptional profiling data.

(a) Flowchart illustrating probe-level filtering. The geometric probe ratio is defined as the geometric mean of one probe’s counts from all segments divided by the geometric mean of all probe counts covering the target(gene) from all the segments in the dataset. (b) Barplot depicting total number of probes remaining in the dataset if different thresholds are selected (1%, 2.5%, 5%, 10%, 20%, 30%, and 50%).