Extended Data Fig. 4: CD300ld-PS Interaction Fails to Induce Intracellular Signaling Axis.
a: RT-qPCR detect CD300ld (left) and S100A8 (right) mRNA expression in neutrophils isolated from mouse bone marrow and treated with B16-F10 tumor explant supernatant (TES), PC liposomes and PS liposomes (a, n = 3 independent samples per group). b: Western blot detection of phosphorylated STAT3 (p-STAT3) in neutrophils treated with TES, PC liposomes and PS liposomes. c-d: Suppression of CD8+ T cell proliferation by splenic PMN-MDSCs pre-treated with TES, PC liposomes and PS liposomes. Representative flow cytometry plots (c) and quantification (d) are shown (d, n = 3 independent samples per group). e: Flow cytometry analysis of CD300ld-ECD-Fc or Fc binding to cells from B16-F10 tumor single-cell suspensions (n = 6 independent samples per group). f: B16-F10 tumor cells were treated with CPT (30 µM) and apoptosis were detected by Flow. g: Engulfment of CellTrace Violet (CTV)-labeled apoptotic B16-F10 cells by WT and KO PMN-MDSCs, shown as representative dot plots (left) and quantification (right) (g, n = 4 independent samples per group). Data are presented as mean ± s.e.m. Statistical analysis was performed using ordinary one-way ANOVA. Data are representative of two (f) or three (a, b, c, d, e, g) independent experiments.
