Extended Data Fig. 4: scRNA-seq of iron-treated CAR-T cells.
From: Iron-mediated ferroptosis impairs CAR-T cell function and antitumor efficacy
a-c, Violin plots displaying distributions of UMI counts (nCount_RNA, a), gene counts (nFeature_RNA, b), and the percentage of mitochondrial gene content (percent_mt, c) after quality control filtering. d, t-SNE plot of all single cells from the three indicated CAR-T treatment groups; the numbers of cells analyzed in each condition are indicated. e, Dot plot showing average expression levels and expression frequency (Pct.exp) of selected marker genes across eight identified clusters; color intensity reflects expression abundance. f, Differential gene expression analysis across all eight clusters comparing CAR-T + FC with CAR-T. Red and green dots indicate adjusted P values < 0.01 and > 0.01, respectively. g, Cytotoxicity scores visualized on t-SNE plots of CAR-T, CAR-T + FC and CAR-T + FC+Fer-1 cells. h and i, Gene expression profiles of iron transport/storage-related genes (h) and ferroptosis-related genes (i) across different clusters in CAR-T and CAR-T + FC groups. The numbers of cells contributing to each cluster are indicated. One sample was analyzed per condition. Violin plots show the distribution of the data; embedded box plots show the median (centre line), 25th and 75th percentiles (box bounds), and minimum to maximum values (whiskers) (a-c, h-i).
