Fig. 5: Photovoltaic epiretinal receptive fields.

a Sketch of the temporal pattern used for stimulation. Each of the 19 pixels centred around the recording location was successively illuminated following a counterclockwise pattern (560 nm, 10 ms, 0.9 mW mm⁻²). The total illumination sequence was repeated for ten consecutive sweeps. The photovoltaic RFs were obtained by two-dimensional Gaussian approximation of the network-mediated ML responses elicited by single-pixel, averaged over sweeps, and normalised to the maximal responding pixel. b Gaussian mixture model of the photovoltaic RF sizes over the small (n = 24 RGCs, blue) and large RGC populations (n = 5 RGCs, red). The average photovoltaic RF diameter of the small and large cells types are, respectively, of 153.7 ± 26.1 µm and 335.5 ± 49.3 µm (mean ± s.e.m.). c Quantification of the number of pixels able to induce statistically significant (P < 0.05) ML activation in the recorded RGC. The horizontal grey line is the median, the red plus is the average and the boxes extend from the 25th to 75th percentiles. d–f Photovoltaic RFs from three individual RGCs classified as small RF cell (d), large RF cell (e) and elongated RF cell (f). The bottom panels show raw electrophysiological recordings and raster plots from the same cells for each single-pixel illumination (560 nm, 10 ms, 0.9 mW mm⁻², first sweep). The red boxes show the pixels inducing a statistically significant activation of the recorded RGC.