Fig. 6: Two-point discrimination pattern reversal test with single-pixel stimulation.

a Sketch of the single-pixel repeated stimulation paradigm. One pixel was repeatedly illuminated at 5 Hz (560 nm, 10 ms, 0.9 mW mm⁻²) for 2 s. b Raw electrophysiological recordings of a single RGC under 5 Hz continuous stimulation from a single pixel. The green triangles correspond to the onset of each pulse. The red arrows highlight the ML activity. c Quantification of the ML firing rate (mean ± s.e.m.) under continuous stimulation from a single pixel (n = 12 RGCs). The grey dashed line is the average resting firing rate with its s.d. represented by the grey area. d Sketch of the two-point discrimination pattern reversal paradigm. The central (orange) pixel was repeatedly illuminated for 1 s at 5 Hz (560 nm, 10 ms, 0.9 mW mm⁻²), then the illumination was switched to the adjacent pixel (purple) for 1 s at 5 Hz (560 nm, 10 ms, 0.9 mW mm⁻²). e Raw electrophysiological recordings of a single RGC under 5 Hz two-point discrimination pattern reversal paradigm. The green triangles correspond to the onset of each light pulse. The pixel switch is highlighted by the purple dashed line. The red arrows highlight ML activity. f Quantification of the ML firing rate (mean ± s.e.m.) under the two-point discrimination pattern reversal paradigm (n = 12 RGCs). The cyan dashed line is the average desensitised ML firing rate and the red dashed line is the recovery threshold. The grey dashed line is the average resting firing rate with its s.d. represented by the grey area.