Fig. 4: Nanochitin physicochemical characterization.

a FTIR spectra of α-chitin (black line), CNCh produced by oxidative cleavage of α-Chitin with LFACA-LPMO (LFACA-EnCNCh, red line), BtLPMO (BtEnCNCh, blue line) and hydrochloric acid (AcCNCh, yellow line). Spectra from 4000 to 750 cm⁻¹. b details of the significant incipient peaks of CNCh at 1740 cm⁻¹, 1621 cm⁻¹, and 1030 cm⁻¹. c CP/MAS ¹³C NMR spectra of α-chitin (black line), LFACA-EnCNCh (red line) produced with LFACA-LPMO enzymatic oxidative cleavage, BtEnCNCh (blue line) produced with extant BtLPMO oxidative cleavage and AcCNCh (yellow line) produced by hydrochloric acid hydrolysis. Inset in the region 178 ppm. d Thermogravimetric analysis curves of α-Chitin (black line), LFACA-EnCNCh (red line) produced with LFACA-LPMO enzymatic oxidative cleavage, BtEnCNCh (blue line) produced with BtLPMO enzymatic oxidative cleavage and AcCNCh (yellow line) produced by hydrochloric acid hydrolysis. Inset of weight loss curves and derivative from TGA curves. e XRD diffractogram for crystalline α-chitin (black line), LFACA-EnCNCh (red line), BtEnCNCh (blue line), AcCNCh (yellow line).