Fig. 3: Photothermal heating.

A, B Internalization rate of both types of Pt nanoparticles, NS (A) and NR (B) in glioblastoma cells as a function of Pt concentration during the 24 h incubation, from [Pt] = 0.2–1.25 mm, measured by ICP-AES on 200,000 cells and renormalized per cell, finally expressed in pg of Pt per cell. C, D Typical photos of spheroids made of 200,000 cells loaded with NSs (C) or NRs (D) incubated at [Pt] = 0.7 mM (left image) or 1.25 mM (right image). E, F IR images upon laser irradiation (808 nm) of different spheroids with increasing Pt loads, indicated as the concentration within the tube in mM of Pt. G, H Temperature elevation reached for the different NS (E) and NR (F) loaded spheroids, dispersed in 5 µL, as a function of the total Pt concentration in this volume. Heating was also measured for 5 µL of aqueous dispersions (solutions) of NS (E) and NR (F) at different Pt concentration.