Fig. 5: In vivo PA imaging of mouse brain vasculature. | Communications Materials

Fig. 5: In vivo PA imaging of mouse brain vasculature.

From: Sharp-peaked lanthanide nanocrystals for near-infrared photoacoustic multiplexed differential imaging

Fig. 5

a Baseline PA image at 799 nm of the mouse brain, using hemoglobin as the contrast agent. b Differential PA image of the mouse brain acquired between 799 nm and 794 nm after injection of Nd(OH)3 nanocrystals, using both hemoglobin and lanthanide-doped nanocrystals as contrast agents, revealing more detailed vasculature. c Overlaid image of the mouse brain after linear unmixing. Green represents hemoglobin contrast and magenta represents the lanthanide nanocrystal contrast. d Contrast-to-noise ratio plotted before and after Nd(OH)3 injection. e Line profiles along the yellow lines in a and b, illustrating the improved visibility of cortical vessels due to Nd(OH)3 injection. f Time course of PA amplitude at 794 nm and 799 nm, along with the normalized differential signal, before, during, and after injection of Nd(OH)3. g Fractional PA amplitude change (799 nm) throughout the experiments. Bar graphs represent the respective mean and standard deviation (s.d.).

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