Fig. 1: Protein encapsulating nanoparticle (NP).
From: Scaleable production of highly loaded protein nanoparticles for immune modulation
a Core comprises protein and DNA in PLG matrix. Shell comprises deoxycholate and E. coli total lipid extract (ECPL). b Schematic of process steps for NP precipitation and post processing. c Visualization of Tequila sunrise layered fluid system for PLG NP precipitation. The stir bar in (1) is 5 mm as a scale calibration. In this demonstration experiment the IPA antisolvent phase (1) contains green fluorescein dye. The dense DMSO layer (2) containing PLG and DNA with red Eosin Y dye is layered under the antisolvent layer. The low-speed impeller is placed at the interface of the two fluids then stirred (3–5). d a representative particle size distribution from precipitation is shown, with an average NP size of 305 nm and polydispersity index (PDI) of 0.1. e SEM of representative NP dispersion, with 1 micron scale bar. f PLG NPs trigger activation of mouse and human TLR2,4 and 9 as determined in TLR-transfected HEK293 reporter cells through measurement of NF-κB activity. Error bars are ± standard deviation of triplicate replicates.
