Fig. 1: Conceptual representation of seasonal and experimental variation in soil moisture in a lowland Amazonian rain forest on sandy soil.

a The amplitude of natural seasonally occurring variation in soil moisture content (control, blue) contrasted with that of the drought treatment (~50%) after 14 years of throughfall exclusion in a lowland evergreen rain forest in Amazonia. Soil moisture in the drought treatment during the rainy season (orange) is within the range of naturally occurring variation (referred to as non-extreme drought); however, it falls below this range in the dry season (extreme drought, red). The natural inter-annual variability in soil moisture is represented by the sinusoidal curve in grey (non-extreme drought). Values indicate the average percentage of soil moisture in control and treatment at the time of soil sampling. b Average annual monthly rainfall and standard deviation measured at the study site during the period 2001–2016 (filled) and for the year 2016 (unfilled). Grey dotted line represents the monthly precipitation required for hydrological balance, considered 100 mm in Amazonia.