Fig. 5: Average nifH gene copies of non-cyanobacterial key diazotrophs assessed by quantitative PCR.

Error bars represent the standard deviation for samples with amplification of two or more replicates. Station numbers on the x-axis are followed by the size fraction (L (large >2 μm) or S (small 0.22–2 μm)) and replicate (A–C). Only data above the limit of detection (LOD) is presented. qPCR assay efficiencies were 105% (Beta-Arctic1), 110% (Gamma-Arctic1), and 107% (Gamma-Arctic2).