Fig. 5: High-sensitivity calcium imaging of large neural volumes.
From: Spatial redundancy transformer for self-supervised fluorescence image denoising
a, Three-dimensional visualization of the neural activity of a 510 × 510 × 200 μm3 volume (100 planes, 0.3 Hz volume rate) in the mouse cortex. Left: the original low-SNR volume. Right: the same volume denoised with SRDTrans. Magnified views of yellow boxed regions are shown under each snapshot. Scale bars, 100 μm for the whole FOV and 10 μm for magnified views. b, Raw frames and SRDTrans denoised counterparts of a single imaging plane at two different moments. The x–z and y–z cross-sections of the volume are shown alongside each x–y plane. Magnified views of yellow boxed regions are shown at the bottom right of the images. Scale bars, 70 μm for the whole FOV and 20 μm for magnified views. c, Fluorescence traces (F) extracted from all pixels on the yellow dashed line in b. Left: traces extracted from raw data. Right: traces extracted from SRDTrans denoised data. Yellow arrowheads point to some representative calcium transients.
