Fig. 6: Regulatory differences between right-sided and left-sided colorectal cancer epithelial cells.
a, Diagram illustrating the left and right sides of the intestines, with the respective number of samples for each group. b, Volcano plot showing differences in transcription factor activity between right-sided and left-sided colorectal cancer epithelial cells. c, Top 10 most active transcription factors identified in epithelial cells from left-sided colorectal cancer based on n = 33 biologically independent samples. The dataset includes 22 samples from the left side (L) and 11 from the right side (R). d, Top 10 most active transcription factors identified in epithelial cells from right-sided colorectal cancer based on n = 33 biologically independent samples. The dataset includes 22 samples from the left side (L) and 11 from the right side (R). e, Differences in patient survival rates based on NFKB2 expression in patients with primary colorectal cancer. f, Consistent differences in gene expression for the ZND350 transcription factor in the TCGA data and our own dataset. g, Consistent differences in gene expression for the NFKB2 transcription factor in two independent patient cohorts. Expression levels are reported in fragments per kilobase of transcript per million mapped reads (FPKM) and counts per million (CPM), respectively. In the boxplots, the line within the box represents the median, and the box extends from the median ± 1.5 times the IQR. Whiskers indicate the 5th and 95th percentiles, and individual sample values are represented as dots. P values were calculated using a two-sided t-test: *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001.
