Table 1 Mock communities used in the present study.

Name	Seq. facility^b	Gene region	Replicates	No. species	No. raw reads	No. sequences after processing	Total no. taxa^d (no filtering)	1st spurious taxon^d (% rel. abundance)	Reference
Mock-1	See ref.	V4	1	27	153,841	140,397	432	0.007	[50]
Mock-2	See ref.	V4	1	58	593,868	578,569	761	0.439	[51]
Mock-3	See ref.	V4	1	21	1,012,097	453,215	1081	0.031	[52]
Mock-4	See ref.	V4	1	14	169,516	159,352	417	0.020	[53]
Mock-5	See ref.	V4	1	21	613,091	108,414	802	0.439	[52]
Mock-6	See ref.	V4	1	21	602,819	231,685	732	0.160	[52]
Mock-7	See ref.	V4	1	20	306,773	42,746	95	0.008	[40]
Mock-TUM	1	V3-V4	7	13	25,640 ± 8516	19,882 ± 8163	77 ± 15	0.130 ± 0.138	This study
ZymoBIOMICS (cat. #D6300)^a	1	V3-V4	7	8^c	67,465 ± 31,752	52,079 ± 24,759	177 ± 33	0.059 ± 0.026	This study
ZymoBIOMICS (cat. #D6311)^a	2	V3-V4 and V4	25	8^c	16,093 ± 9319	14,383 ± 9083	17 ± 15	0.106 ± 0.085	This study

In case of replicates, data are shown as mean ± SD.
The sequences of all species included in the respective mock communities are available via the project-specific data repository: https://doi.org/10.5281/zenodo.4837436.
^aWhile D6300 corresponds to an evenly distributed mixture of the microbes, D6311 is a log-distributed mixture of DNA from the same microbes.
^bFor studies from the literature, please refer to the corresponding listed reference. For in-house generated data in this study: 1, ZIEL Core Facility Microbiome, TU Munich, Freising, Germany; 2, Joint Microbiome Facility of the Medical University of Vienna and the University of Vienna (JMF), Austria.
^cBacterial species only. The mock community includes also two yeast species not considered in the present study (ten microbial species in total).
^dAll values refer to OTUs clustered at 97% sequence identity, with exception of the last mock community analyzed in sequencing facility 2, for which values refer to ASVs.

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