Fig. 1: Culture-dependent isolate analysis of captive Panamanian golden frogs.

a Phylogenetic tree of bacteria cultured from the skin of 18 golden frogs screened for the presence of known anti-Bd bacteria. Bars represent strength of anti-Bd inhibition for a subsample of candidate probiotics identified from an existing database, isolates used in the final probiotic experiment are highlighted in red. b Inhibitory isolates selected for the probiotic consortium, note the genetically modified Diaphorobacter 63F:vio was included in the probiotic cocktail treatment. **The core skin microbe Diaphorobacter 63F wild-type strain was not used in probiotic consortium, but is shown here to illustrate the colony color prior to genetic modification.