Fig. 1: Examples of study design for the sampling of microbes of symbiotic organisms, their hosts and environment. | ISME Communications

Fig. 1: Examples of study design for the sampling of microbes of symbiotic organisms, their hosts and environment.

From: MIxS-SA: a MIxS extension defining the minimum information standard for sequence data from symbiont-associated micro-organisms

Fig. 1

a Trematode Coitocaecum parvum different life stages (S1, S2, S3, S4) are reported with the MIxS-SA package. The microbiome of infected snail (H1), amphipod (H2) and fish (H3) hosts are reported with the MIxS host-associated package. In addition, the microbiome of environmental sediment (E1) and water (E2) from which these organisms were collected can be reported with MIxS-sediment and MIxS-water, respectively. The following relationships are reported: S1 “within” H1, S2 “within” E2, S3 “within” H2, S4 “within” H3, H1 “next to” E1, H1 “within” E2, H2 “within” E2, H3 “within” E2, E1 “next to” E2. b Angiosperm Orobanche hederae (S5, S6) parasitizing a host plant (P1, P2, P3) is reported using the included MIxS-SA and MIxS-PA (plant-associated) packages respectively. In addition, the MIxS-soil package is used to report corresponding soil samples. The following relationships are reported: S6 ‘within’ P1, S5 ‘next to’ S6, P1 ‘next to’ P2, P1 ‘next to’ P3, P2 ‘next to’ P3, P1 ‘within’ E3, P3 ‘within’ E4, E3 ‘next to’ E4.

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