Table 1 MIxS symbiont-associated environmental package representative terms, along with requirement status, description and MIXS IDs.

From: MIxS-SA: a MIxS extension defining the minimum information standard for sequence data from symbiont-associated micro-organisms

MIxS Package

Metadata category

Package item

Req

Definition

MIXS ID

Symbiont-associated

Symbiont specific descriptors

host dependence

M

Type of host dependence for the symbiotic host organism to its host.

0001315

type of symbiosis

C

Type of biological interaction established between the symbiotic host organism being sampled and its respective host.

0001307

symbiotic host organism life cycle type

M

Type of life cycle of the symbiotic host species (the thing being sampled). Simple life cycles occur within a single host, complex ones within multiple different hosts over the course of their normal life cycle.

0001300

host life stage

M

Description of life stage of host.

0000251

mode of transmission

C

The process through which the symbiotic host organism entered the host from which it was sampled.

0001312

route of transmission

O

Description of path taken by the symbiotic host organism being sampled in order to establish a symbiotic relationship with the host (with which it was observed at the time of sampling) via a mode of transmission (specified in mode_transmission).

0001316

host number individual

O

Number of symbiotic host individuals pooled at the time of collection.

0001305

Symbiont – host relationship descriptors

observed host symbionts

O

The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host. For cases when the specific host of the sample is a symbiont this field should refer to other organisms it is associated with: e.g.: hyperparasite species X (parasite of the parasite).

0001309

host specificity

C

Level of specificity of symbiont-host interaction: e.g. generalist (symbiont able to establish associations with distantly related hosts) or species-specific.

0001308

host of the symbiont role

C

Role of the host in the life cycle of the symbiotic organism.

0001303

host cellular location

C

The localization of the symbiotic host organism within the host from which it was sampled: e.g., intracellular if the symbiotic host organism is localized within the cells or extracellular if the symbiotic host organism is localized outside of cells.

0001313

duration of association with the host

O

Time spent in host of the symbiotic organism at the time of sampling; relevant scale depends on symbiotic organism and study.

0001299

observed coinfecting organisms in host of host

O

The taxonomic name of any coinfecting organism observed in a symbiotic relationship with the host of the sampled host organism. e.g. where a sample collected from a host trematode species (A) which was collected from a host_of_host fish (B) that was also infected with a nematode (C), the value here would be (C) the nematode {species name} or {common name}. Multiple coinfecting species may be added in a comma-separated list. For listing symbiotic organisms associated with the host (A) use the term Observed host symbiont.

0001310

Host of the symbiont descriptors

host of the symbiotic host common name

O

Common name of the host of the symbiotic host organism.

0001324

host of the symbiotic host local environmental context

O

For a symbiotic host organism the local anatomical environment within its host may have causal influences. Report the anatomical entity(s) which are in the direct environment of the symbiotic host organism being sampled and which you believe have significant causal influences on your sample or specimen. For example, if the symbiotic host organism being sampled is an intestinal worm, its local environmental context will be the term for intestine from UBERON (http://uberon.github.io/).

0001325

host of the symbiotic host environmental medium

O

Report the environmental material(s) immediately surrounding the symbiotic host organism at the time of sampling. This usually will be a tissue or substance type from the host, but may be another material if the symbiont is external to the host. We recommend using classes from the UBERON ontology, but subclasses of ‘environmental material’ (http://purl.obolibrary.org/obo/ENVO_00010483) may also be used. EnvO documentation about how to use the field: https://github.com/EnvironmentOntology/envo/wiki/Using-ENVO-with-MIxS. Terms from other OBO ontologies are permissible as long as they reference mass/volume nouns (e.g., air, water, blood) and not discrete, countable entities (e.g., intestines, heart).

0001326

host of the symbiotic host taxon id

O

NCBI taxon id of the host of the symbiotic host organism.

0001306

host of the symbiotic host subject id

O

A unique identifier by which each host of the symbiotic host organism subject can be referred to, de-identified, e.g. #H14.

0001327

Core

 

Relationship to other samplesa

C

indicates the direct relationship with another sample from the same Bioproject. Accepted terms are: ‘technical replicate of’, ‘after’, ‘before’, ‘next to’, ‘within’, and ‘contains’. Can be repeated to reveal relationship to many samples.

TBD

  

Biotic relationship

C

Description of relationship(s) between the subject organism and other organism(s) it is associated with. E.g., parasite on species X; mutualist with species Y. The target organism is the subject of the relationship, and the other organism(s) is the object.

0000028

Otherb

 

Observed host symbionts

O

The taxonomic name of the organism(s) found living in mutualistic, commensalistic, or parasitic symbiosis with the specific host.

0001309

  1. Req requirements, O optional, C conditional, M mandatory, TBD to be determined.
  2. aTerm may be slightly amended when included to all packages.
  3. bTerm added to host-associated, human-associated, plant-associated, human-vaginal, human-skin, human-oral, and human-gut packages.