Fig. 3: Comparison of supervised classification of flow cytometry data and 16S rRNA gene sequencing on mock communities. | ISME Communications

Fig. 3: Comparison of supervised classification of flow cytometry data and 16S rRNA gene sequencing on mock communities.

From: Fast quantification of gut bacterial species in cocultures using flow cytometry and supervised classification

Fig. 3

A Expected proportions and proportions predicted with CellScanner for R. intestinalis (RI) and E. coli (EC), the latter expressing mCherry. 16S rRNA gene sequencing results are included. B Expected proportions and proportions predicted with CellScanner of FP (F. prausnitzii) and EC, the latter expressing mCherry. C Expected proportions and proportions predicted with CellScanner, without using the red channels in the classification, of RI and EC expressing mCherry. 16S rRNA gene sequencing data are included. D Expected proportions and proportions predicted with CellScanner, without using the red channels in the classification, of FP and EC expressing mCherry. All flow cytometry shown here were obtained with CytoFLEX.

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