Fig. 4: Proportions in two- and three-species mock communities predicted through supervised classification of flow cytometry data. | ISME Communications

Fig. 4: Proportions in two- and three-species mock communities predicted through supervised classification of flow cytometry data.

From: Fast quantification of gut bacterial species in cocultures using flow cytometry and supervised classification

Fig. 4

A Intended and predicted proportions for Bact. thetaiotaomicron (BT) and C. aerofaciens (CA). In contrast to expected proportions, intended proportions have not been corrected for pipetting errors (see Methods). Error bars: Standard deviation over two technical replicates. Flow cytometry was carried out with Accuri C6. B Expected proportions and proportions predicted by CellScanner for R. intestinalis (RI) and F. prausnitzii (FP). 16S rRNA gene sequencing data are included. C Proportions predicted by CellScanner for a three-species mock community of R. intestinalis, E. coli and Bact. uniformis. D Confusion matrix of an in silico three-species community made from the monocultures of the species in (C). For panels (B–D), flow cytometry was carried out with CytoFLEX.

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