Table 1 Characteristics of species used in this study.

From: Fast quantification of gut bacterial species in cocultures using flow cytometry and supervised classification

Strain

Gram

Size (length – width, μm)

Shape & Occurrence

Phylum, family

Reference (Species size)

16S rRNA copy number

Bacteroides thetaiotaomicron

DSM 2079

1.0–2.0 × 0.7–1.0

Oval bacillus

Single or pairs

Bacteroidetes, Bacteroidaceae

Eggerth et al. 1932 [63]

5

Bacteroides uniformis

RCC 1835

0.8 × 1.5

Bacillus

Single

Bacteroidetes, Bacteroidaceae

Eggerth et al. 1932 [63]

4

Bifidobacterium adolescentis

RCC 1139

+

4.0–6.0 × 1.0

Y or bifid

Actinobacteria, Bifidobacteriaceae

Kim et al. 2010 [64]

4

Bilophila wadsworthia

RCC 662

0.7–1.1 × 1.0 -10.0

Bacillus

Pleomorphic

Proteobacteria, Desulfovibrionaceae

Baron et al. 1989 [65]

4

Blautia hydrogenotrophica

DSM 10507

+

0.6–0.7

Coccobacillus

Pairs

Firmicutes, Lachnospiraceae

Bernalier et al. 1996 [56, 66]

1

Collinsella aerofaciens

RCC 1366

+

0.3–0.7 × 1.2 - 4.3

Cocci

Chain of 6-120 cells

Actinobacteria, Coriobacteriaceae

Kageyama et al. 1999 [67]

5

Escherichia coli*

DSM 6050

-

1.0–2.0 × 0.5

Bacillus

Single

Proteobacteria, Enterobacteriaceae

Riley, 1999 [68]

7

Faecalibacterium prausnitzii

DSM 17677

0.5–0.8 × 2.0–9.0

Bacillus

Firmicutes, Clostridiales

Duncan et al. 2002 [69]

6

Parabacteroides merdae

RCC 643

0.8–1.6 × 1.2–12

Bacillus

Single

Bacteroidetes, Porphyromonadaceae

Sakamoto et al. 2006 [70]

7

Prevotella copri

DSM 18205

0.83 × 0.91–0.99

Bacillus

Single

Bacteroidetes, Prevotellaceae

Hayashi et al. 2007 [71]

4

Roseburia intestinalis

DSM 14610

Gram variable(+)

0.5 × 1.5–3.0

Bacillus

Firmicutes, Lachnospiraceae

Duncan et al. 2002 [72]

6

Ruminococcus bromii

RCC 1377

+

0.7–1.1(diameter)

Cocci

Pairs and chains up to 8 cells

Firmicutes, Ruminococaceae

Moore et al. 1972 [73]

4

  1. DSMZ: German Collection of Microorganisms and Cell Cultures GmbH. RCC: Raes lab Culture Collection. Cell size and shape are reported for different media from literature. Cell size is known to be variable depending on growth phase and medium used. 16S rRNA copy numbers were taken from rrnDB [44]. Bacillus shape: rod-like. Coccus shape: Sphere-like.
  2. * With and without plasmid pRSetB-mCherry.