Fig. 1: Experimental setup. | ISME Communications

Fig. 1: Experimental setup.

From: Repeated introduction of micropollutants enhances microbial succession despite stable degradation patterns

Fig. 1

In microcosm batch 1 (B1), 600 ml of the initial inoculum was added to bottles containing the micropollutants. Starting from the B1, 60 mL (10%) of the inoculum was pipetted from each of the replicates and transferred to fresh medium containing 540 mL of 0.22-μm-filtered water and freshly added micropollutants every 5 days. This procedure was carried out in the seven batches of microcosms from batch 1 (B1) through batch 7 (B7). Samples for bacterial community characterization and micropollutant removal were collected prior to the next inoculation. Treatments are color-coded: “Control” is (without micropollutant addition, gray), “BPA” (microcosms added with bisphenol A, red), “TCS” (microcosms added with triclosan, blue), “MI” (microcosms added with a mixture containing bisphenol A and triclosan, purple), “MII” (microcosms added with a mixture containing bisphenol A and triclosan, bisphenol S and triclocarban, yellow).

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