Fig. 2: Determination of phosphorus-releasing capacity of pure-cultured bacteria that include B. subtilis H6, E. coli BL21, B. megaterium ACCC02970, A. hydrophila ATCC7966, B. cepacian BN337012, and E. faecium PH07. | ISME Communications

Fig. 2: Determination of phosphorus-releasing capacity of pure-cultured bacteria that include B. subtilis H6, E. coli BL21, B. megaterium ACCC02970, A. hydrophila ATCC7966, B. cepacian BN337012, and E. faecium PH07.

From: Single-cell Raman-activated sorting and cultivation (scRACS-Culture) for assessing and mining in situ phosphate-solubilizing microbes from nature

Fig. 2

A Halo zones produced by these strains on the solid medium of MM-iiP and MM-oiP, respectively; B Concentrations of soluble P in supernatant via the molybdenum-antimony resistance colorimetric method in the liquid medium of MM-iiP and MM-oiP, respectively. Error bars represent the standard deviation of three measurements from biological triplicates; C C-D ratios of the six bacteria strains incubated in minimum media containing 50%-D2O without P (P_free), with dissolvable P (sP), Ca3(PO4)2 (iiP) and lecithin (oiP), respectively. CDR distribution patterns of the six species are compared between MM-iiP (D) and MM-oiP (E). CDR = 0.20 was chosen as the threshold. Sixty individual cells from each bacteria sample were measured by Raman spectroscopy.

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